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Enzymatic transglycosylation of natural and modified nucleosides by immobilized thermostable nucleoside phosphorylases fromGeobacillus stearothermophilus

✍ Scribed by S. A. Taran; K. N. Verevkina; S. A. Feofanov; A. I. Miroshnikov

Book ID: 111457036
Publisher: SP MAIK Nauka/Interperiodica
Year: 2009
Tongue: English
Weight: 181 KB
Volume: 35
Category: Article
ISSN: 1068-1620
DOI: 10.1134/s1068162009060107

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## Abstract Multimeric uridine phosphorylase (UP) and purine nucleoside phosphorylase (PNP) of __Bacillus subtilis__ have been expressed from genes cloned in __Escherichia coli__, purified, characterized, immobilized and stabilized on solid support. A new immobilization strategy has been developed