Enzymatic synthesis of the precursor of Leu-enkephalin in water-immiscible organic solvent systems
β Scribed by Yukitaka Kimura; Kazuhiro Nakanishi; Ryuichi Matsuno
- Publisher
- Elsevier Science
- Year
- 1990
- Tongue
- English
- Weight
- 914 KB
- Volume
- 12
- Category
- Article
- ISSN
- 0141-0229
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Enzymatic syntheses ofbioacitive pentapeptide, N-protected Leu-enkephalin and some other oligopeptides in organic solvents were studied. The stereoselectivity of the enzymatic reaction was examined by using racemic substrates. Different enzymes, solvent systems and protecting groups were compared. T
Full enzymatic synthesis of a fragment of osteogenic growth peptide (OGP), a precursor of bioactive pentapeptide OGP(10-14) (Z-TyrGlyPheGlyGlyOEt), was accomplished by papain, a-chymotrypsin, and thermolysin via 2+3 or 3+2 synthetic routes in organic solvents for the first time. The factors influenc