Enzymatic Synthesis and Purification of
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Till Beuerle; Eran Pichersky
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Article
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2002
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Elsevier Science
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English
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Two recombinant His-tagged proteins, a plant 4-coumarate:coenzyme A ligase (EC 6.2.1.12) and a bacterial benzoate:coenzyme A ligase (EC 6.2.1.25), were expressed in Escherichia coli and purified in a single step using Ni-chelating chromatography. Purified enzymes were used to synthesize cinnamoyl-co