Enzymatic synthesis of manno- and heteromanno-oligosaccharides using α-mannosidases: A comparative study of linkage-specific and non-linkage-specific enzymes
✍ Scribed by Sony Suwasono; Robert A. Rastall
- Publisher
- Wiley (John Wiley & Sons)
- Year
- 1998
- Tongue
- English
- Weight
- 239 KB
- Volume
- 73
- Category
- Article
- ISSN
- 0268-2575
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✦ Synopsis
Two a-mannosidase enzymes (EC 3.2.1.24) and an a-1,2-mannosidase enzyme (EC 3.2.1.113) were compared for their ability to synthesise heterooligosaccharides by the equilibrium approach (condensation reaction). A panel of disaccharide acceptors was used and product yields and product spectra determined. Choice of enzyme had a signiÐcant inÑuence on product yield, with yield being dependent on enzyme and substrate combination. Most of the enzyme/ substrate combinations tested gave 1 ] 6 linked manno-oligosaccharides as the principal product. Mannose was linked to lactose, cellobiose and lactulose by both 1 ] 3 and 1 ] 6 linkages in ratios that depended upon the enzyme used. Homo-oligosaccharide (mannotriose) formation was found in all but one of the enzyme/substrate combinations tested and, in the case of the a1,2-mannosidase, the structure of the mannotrioses depended upon the acceptor used. Formation of hetero-oligosaccharides over homo-oligosaccharides could be favoured by use of low mannose : acceptor ratios.