Enzymatic synthesis of Leu- and Met-enkephalin
β Scribed by Willi Kullmann
- Book ID
- 118313742
- Publisher
- Elsevier Science
- Year
- 1979
- Tongue
- English
- Weight
- 304 KB
- Volume
- 91
- Category
- Article
- ISSN
- 0006-291X
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## Abstract This paper describes the total enzymatic synthesis of Leuβenkephalin (TyrβGlyβGlyβPheβLeu) in which all residues were labelled with tritium. Carboxypeptidase Y from __Saccharomyces cerevisiae__ was the coupling enzyme. [^3^H]βTyrβNH~2~, [^3^H]βGlyβOet, [^3^H]βPheβNH~2~ and [^3^H]βLeuβNH
## Abstract The synthesis is described of several peptides containing the sequence PheβMet. In order to limit enzymatic degradation and to restrict the number of possible conformations they were cyclized with variable ring size. Some unexpected sideβreactions were encountered during the synthesis o