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Enzymatic synthesis of glutathione using yeast cells in two-stage reaction

✍ Scribed by Wei Li; Zhimin Li; Qin Ye


Book ID
106146963
Publisher
Springer
Year
2009
Tongue
English
Weight
382 KB
Volume
33
Category
Article
ISSN
1615-7605

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A sensitive and specific assay for glutathione using a recycling reaction followed by spectrophotometric detection in a flow-injection analysis system is presented. The proposed method provides specific amplification of the response to glutathione by combined use of the enzyme GSSG reductase and the