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Enzymatic Synthesis of Galactose-Containing Disaccharides Employing β-Galactosidase from Bacillus circulans

✍ Scribed by Erzsébet Farkas; Joachim Thiem

Publisher
John Wiley and Sons
Year
1999
Tongue
English
Weight
326 KB
Volume
1999
Category
Article
ISSN
1434-193X

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✦ Synopsis

Galβ1Ǟ4 disaccharide structures are vital core units of the rides {Galβ1Ǟ4GlcNAcαOAll (3), Galβ1Ǟ4GlcNAcβOAll (5), Galβ1Ǟ4GalαOAll (10), Galβ1Ǟ4GalβOAll (12), Galβ1Ǟ oligosaccharide components of glycoconjugates. β-Galactosidase from Bacillus circulans (E.C.3.2.1.23) catalyses the transfer 4GalβSPh ( 14), Galβ1Ǟ4GalβOpNP ( 16) and the trisaccharide Galβ1Ǟ4Galβ1Ǟ4GalβOpNP (18)} has been achieved in 30-66 of galactose from a donor structure such as GalβOpNP to various GlcNAc and galactose derivatives, forming β1Ǟ4 lin-% yield by application of this enzyme. kages. The synthesis of several biologically relevant disacchaases to oligosaccharide synthesis has been restricted by their of the desired disaccharide product. The optimised reaction [a]

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✍ M. A. Boon; A. E. M. Janssen; A. van der Padt 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 253 KB 👁 2 views

The aim of this research is to develop a model to describe oligosaccharide synthesis and simultaneously lactose hydrolysis. Model A (engineering approach) and model B (biochemical approach) were used to describe the data obtained in batch experiments with ␤-galactosidase from Bacillus circulans at v