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Enzymatic synthesis of dodecyl.β-d-glucopyranoside catalyzed by Candida molischiana 35M5N β-glucosidase

✍ Scribed by Yannick Gueguen; Patrick Chemardin; Patrick Pommares; Alain Arnaud; Pierre Galzy

Publisher: Elsevier Science
Year: 1995
Tongue: English
Weight: 607 KB
Volume: 53
Category: Article
ISSN: 0960-8524
DOI: 10.1016/0960-8524(95)00095-v

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✦ Synopsis

The enzymatic synthesis of dodecyl. /?-o-glucopyranoside was studied by using the j?-glucosidase from

Candida molischiana 35M5N in the examination of trans&coJylation and hydrolysis reversion reactions. On account of cost considerations, the condensation reaction was optimized. The effects of the extraction solvent, the initial I-dodecanol and glucose concentrations, the temperature and the incubation

time were studied. The optimal conditions for the enzymatic synthesis of dodecyl. fl-D-glucopyranoside by the condensation reaction were: 90% (v/v) I-dodecanol initial concentration; glucose concentration (50 mM), temperature 40°C and reaction time 20 h.

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