Enzymatic synthesis of a peptide bond between a tryptic fragment of horse heart cytochrome c and a synthetic model peptide

✍ Scribed by L. W. Westerhuis; G. I. Tesser; R. J. F. Nivard

• Book ID: 104588282
• Publisher: Elsevier Science
• Year: 2010
• Tongue: English
• Weight: 417 KB
• Volume: 99
• Category: Article
• ISSN: 0165-0513
• DOI: 10.1002/recl.19800991207

✦ Synopsis

Abstract

As a model study for the usefulness of the synthetic action of a proteinase in the semisynthesis of large polypeptides, a heme peptide, comprising the residues 1‐38 of horse heart cytochrome‐c and bearing protective functions on the ϵ‐amino groups, was incubated with trypsin in the presence of H‐Orn(Msc)‐Trp‐NHCH~3~ in a mixture of water and organic solvents. We found that the proteolytic enzyme catalysed the formation of a peptide bond between the fragment and the dipeptide derivative.