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Enzymatic Synthesis of a Neoglycoconjugate by Transglycosylation withArthrobacterEndo-β-N-acetylglucosaminidase: A Substrate for Colorimetric Detection of Endo-β-N-acetylglucosaminidase Activity

✍ Scribed by Kaoru Takegawa; Kiyotaka Fujita; Jian-Qiang Fan; Mitsuaki Tabuchi; Naotaka Tanaka; Akihiro Kondo; Hiroyuki Iwamoto; Ikunoshin Kato; Yuan C. Lee; Shojiro Iwahara


Publisher
Elsevier Science
Year
1998
Tongue
English
Weight
153 KB
Volume
257
Category
Article
ISSN
0003-2697

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Analysis of endo-β-N-acetylglucosaminida
✍ Hitoo Iwase; Tetuo Morinaga; Yu-Teh Li; Su-Chen Li 📂 Article 📅 1981 🏛 Elsevier Science 🌐 English ⚖ 235 KB

We have devised a new method for assaying the endo-p-N-acetylglucosaminidase activity by using the dansyl asparaginyl oligosaccharide, (Man),(GlcNAc)z-Asn-DNS, as the substrate and analyzing the product, GlcNAc-Asn-DNS, by a reverse-phase high-pressure liquid chromatography using a silica-based chem