The enzymatic synthesis of the tripeptide derivative Z-Gly-Trp-Met-OEt is reported. This tripeptide is a fragment of the cholecystokinin C-terminal octapeptide CCK-8. Studies on the a-chymotrypsin catalyzed coupling reaction between Z-Gly-Trp-R, and Met-R, have focused on low water content media, us
Enzymatic synthesis of a CCK-8 tripeptide fragment
β Scribed by Hua Xiang; Heiner Eckstein
- Publisher
- John Wiley and Sons
- Year
- 2010
- Tongue
- English
- Weight
- 397 KB
- Volume
- 22
- Category
- Article
- ISSN
- 0256-7660
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The kinetically controlled condensation reaction of Z-Gly-Trp-Met-OR 1 (R 1 : Et, Al, Cam) and H-Asp-(OR 2 )-Phe-NH 2 (R 2 : H, Bu t ) catalyzed by β£-chymotrypsin deposited onto polyamide in organic media was studied. The effect of the drying process of the enzyme-support preparation, substrate conc
## Abstract The enzymatic synthesis of the cholecystokinin octapeptide (CCKβ8) is reported. The target octapeptide CCKβ8 is the minimum active sequence with the same biological activity as naturally occurring cholecystokinin and is a potential therapeutic agent in the control of gastrointestinal fu