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Enzymatic saccharification of cellulose in aqueous-ionic liquid 1-ethyl-3-methylimidazolium dimethylphosphate-DMSO media

✍ Scribed by Yu C. He; Cui L. Ma; Dong Q. Xia; Liang Ding; Liang Z. Li; Mu Xian


Book ID
111491502
Publisher
Versita
Year
2011
Tongue
English
Weight
344 KB
Volume
65
Category
Article
ISSN
0366-6352

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✦ Synopsis


Abstract

Ionic liquid (IL) 1-ethyl-3-methylimidazolium dimethylphosphate ([Emim]DMP) was chosen as an environment-friendly solvent to enzymatically hydrolyze cellulose in situ. Under optimal reaction condition, 80.2 % of cellulose (10 mg mLβˆ’1) were converted to glucose in aqueous-IL-DMSO (Ο† r = 74: 25: 1) media at 55Β°C in 18 h. Finally, fermentability of the recovered hydrolyzates was evaluated using Saccharomyces cerevisiae which is able to ferment hydrolyzates efficiently, the ethanol production was 0.44 g gβˆ’1 of glucose within 24 h of the process. Such information is vital for the saccharification of more complex cellulose materials and for the fermentation of hydrolyzates into biofuel.


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## Abstract **BACKGROUND:** The high crystallinity of cellulose underlies the recalcitrance that this polymer presents in enzymatic degradation. Thus, a pre‐treatment step is applied in most bioconversion processes. Treatments with ionic liquids are considered an emerging pre‐treatment technology,