Enzymatic reduction of complex redox dyes using NADH-dependent reductase fromBacillus subtiliscoupled with cofactor regeneration

Asymmetric Reduction of α-Keto Esters wi

Asymmetric Reduction of α-Keto Esters with Thermus thermophilus NADH-Dependent Carbonyl Reductase using Glucose Dehydrogenase and Alcohol Dehydrogenase for Cofactor Regeneration

✍ Angela Pennacchio; Assunta Giordano; Mosè Rossi; Carlo A. Raia 📂 Article 📅 2011 🏛 John Wiley and Sons 🌐 English ⚖ 155 KB

## Abstract The enantioselective synthesis of methyl (__R__)‐mandelate and methyl (__R__)‐__o__‐chloromandelate was investigated using an NADH‐dependent carbonyl reductase from __Thermus thermophilus__ (TtADH) and, separately, archaeal glucose dehydrogenase and __Bacillus stearothermophilus__ alcoh