✦ LIBER ✦
Enzymatic laboratory scale production of homochiral (R)-3-fluorolactic acid methyl ester via enantiospecific reduction of sodium fluoropyruvate catalyzed by rabbit muscle l-lactate dehydrogenase (l-LDH)
✍ Scribed by L.P.B. Gonçalves; O.A.C. Antunes; G.F. Pinto; E.G. Oestreicher
- Publisher
- Elsevier Science
- Year
- 1996
- Tongue
- English
- Weight
- 279 KB
- Volume
- 7
- Category
- Article
- ISSN
- 0957-4166
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✦ Synopsis
In the present work a simple laboratory procedure for the synthesis of (R)-3-Fluomlactic Methyl Ester(l) is described. A coupled enzymatic system formed by tacit muscle L-lactate dehydrogcnase (L-LDH), horse liver alcohol dehydrogenase (HLADH), fluoropyruvic acid sodium salt, NAD + and cis-l,2-bis(hydroxymethyl)cyclohexane were found to be very effective for production of (1) in 80% overall yield and ee > 99%.