𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Enzymatic introduction of a fluorescent sialic acid into oligosaccharide chains of glycoproteins

✍ Scribed by Hans Jürgen GROSS; Reinhard BROSSMER

Book ID
115126038
Publisher
John Wiley and Sons
Year
1988
Tongue
English
Weight
697 KB
Volume
177
Category
Article
ISSN
1432-1327

No coin nor oath required. For personal study only.

📜 SIMILAR VOLUMES

A micromethod for the estimation of olig
A micromethod for the estimation of oligosaccharides containing glycosidically linked sialic acid or hexoses, or both, in glycoproteins
✍ Steven M. Chamow; Jerry L. Hedrick 📂 Article 📅 1988 🏛 Elsevier Science 🌐 English ⚖ 600 KB

The peeling reaction, the process by which oligosaccharides are degraded in alkali, was used as the basis for an assay to provide structural information about glycosidically linked oligosaccharides in glycoproteins. Glycoproteins were treated with 0.05 M NaOH at 50 degrees to induce release, and sub

Rapid Quantitative Determination of Sial
Rapid Quantitative Determination of Sialic Acids in Glycoproteins by High-Performance Liquid Chromatography with a Sensitive Fluorescence Detection
✍ K.R. Anumula 📂 Article 📅 1995 🏛 Elsevier Science 🌐 English ⚖ 563 KB

Sialic acids were specifically labeled with o-phenylenediamine-2HCl (OPD) to yield stable fluorescent quinoxaline derivatives. The sialic acids were released from the glycoprotein in a NaHSO4 solution (0.25 M 80 degrees C, 20 min) and derivatized in the same solution with the OPD (10 mg/ml (final co