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Enzymatic Glycoprotein Synthesis: Preparation of Ribonuclease Glycoforms via Enzymatic Glycopeptide Condensation and Glycosylation
β Scribed by Witte, Krista; Sears, Pamela; Martin, Richard; Wong, Chi-Huey
- Book ID
- 126788076
- Publisher
- American Chemical Society
- Year
- 1997
- Tongue
- English
- Weight
- 207 KB
- Volume
- 119
- Category
- Article
- ISSN
- 0002-7863
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β€-methylaspartate ammonia-lyase, EC 4.3.1.2, (β€-methylaspartase) from Clostridium tetanomorphum was used to produce a 40/60 molar ratio of (2S,3R) and (2S,3S)-3-methylaspartic acids, 2a and 2b, respectively, from mesaconic acid 1 as substrate, on a large scale. To prepare (3R,4R)-3-methyl-4-(benzylo