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Enzymatic estimation of glycogen in the cleaving mouse embryo

✍ Scribed by Stern, Samuel ;Biggers, J. D.

Publisher: John Wiley and Sons
Year: 1968
Tongue: English
Weight: 368 KB
Volume: 168
Category: Article
ISSN: 0022-104X
DOI: 10.1002/jez.1401680106

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✦ Synopsis

Recently developed enzymatic techniques for the ultramicro determination of glycogen (Bueding and Hawkins, '64; Passonneau et al., '67) were used to estimate the glycogen content of preimplantation mouse embryos. From an initial value of 0.14 mpg glycogen/embryo in the 1-cell fertilized egg. the amount of glycogen increased to 2.24 qpg/embryo at the 8-cell stage. During the next 48 hours of development, the level fell to 1.81 mpg/embryo.

A comparison with previous histochemical findings (Thomson and Brinster, '66) and the possible relationship 01 glycogen concentration and metabolic capabilities of the embryos is discussed.

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