Enzymatic enantioselective C–C-bond formation in microreactors

✍ Scribed by K. Koch; R.J.F. van den Berg; P.J. Nieuwland; R. Wijtmans; H.E. Schoemaker; J.C.M. van Hest; F.P.J.T. Rutjes

• Publisher: John Wiley and Sons
• Year: 2008
• Tongue: English
• Weight: 188 KB
• Volume: 99
• Category: Article
• ISSN: 0006-3592
• DOI: 10.1002/bit.21649

✦ Synopsis

Abstract

We have demonstrated that multiple crude enzyme lysates containing a hydroxynitrile lyase can be used for the enantioselective synthesis of cyanohydrins from aldehydes in microchannels. Using a microreactor setup, two important parameters were efficiently screened consuming only minute amounts of reagents. More importantly, results from the continuous flow reaction were fully consistent with results obtained from larger batchwise processes in which a stable emulsion was formed. Biotechnol. Bioeng. 2008; 99; 1028–1033. © 2007 Wiley Periodicals, Inc.