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Enzymatic determinations of blood galactose with galactose oxidase and galactose dehydrogenase

✍ Scribed by Magnus Hjelm; Bertil Tengström

Book ID
115765891
Publisher
Elsevier Science
Year
1968
Tongue
English
Weight
148 KB
Volume
2
Category
Article
ISSN
0006-2944

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Colorimetric determination of galactose
Colorimetric determination of galactose and galactose-1-phosphate from dried blood
✍ Frank Diepenbrock; Rolf Heckler; Haribert Schickling; Thomas Engelhard; Daniel B 📂 Article 📅 1992 🏛 Elsevier Science 🌐 English ⚖ 240 KB

A colorimetric microassay for the simultaneous quantitative determination of galactose (Gal) and galactose-l-phosphate (Gall-P) in dried blood spots is described. An enzymatic reaction involving alkaline phosphatase (EC 3.1.3.1) and galactose dehydrogenase (EC 1.1.1.48) produces NADH, which is coupl