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Enzymatic determination of l-alanine with ω-amino acid:pyruvate aminotransferase

✍ Scribed by Kazuo Yonaha; Seizen Toyama


Publisher
Elsevier Science
Year
1980
Tongue
English
Weight
190 KB
Volume
101
Category
Article
ISSN
0003-2697

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✦ Synopsis


A simple and specific method with bacterial o-amino acid:pyruvate aminotransferase and lactate dehydrogenase has been reported for the determination of L-alanine. This method involves a transamination of L-alanine with sulfoacetaldehyde to produce pyruvate and the spectrometric determination of this product with the aid of lactate dehydrogenase.


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