Enzymatic determination of l-alanine with ω-amino acid:pyruvate aminotransferase
✍ Scribed by Kazuo Yonaha; Seizen Toyama
- Publisher
- Elsevier Science
- Year
- 1980
- Tongue
- English
- Weight
- 190 KB
- Volume
- 101
- Category
- Article
- ISSN
- 0003-2697
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✦ Synopsis
A simple and specific method with bacterial o-amino acid:pyruvate aminotransferase and lactate dehydrogenase has been reported for the determination of L-alanine. This method involves a transamination of L-alanine with sulfoacetaldehyde to produce pyruvate and the spectrometric determination of this product with the aid of lactate dehydrogenase.
📜 SIMILAR VOLUMES
## Abstract Formal CH~2~ insertion in bioconjugates composed of 1′‐aminoferrocene‐1‐carboxylic acid (Fca) and alanine Boc‐Ala‐Fca‐Ala‐OCH~3~ gives Fca bioconjugates with the β‐amino acid (__S__)‐3‐amino‐2‐methylpropanoic acid (Aib). The novel homologous conjugates of ferrocene were fully characteri
A procedure for the simultaneous preparation of S-sulfo-L-cysteine and L-alanine 3-sulfinic acid is described. The method is based on the quantitative reaction between sulfite and S-(2amino-2-carboxyethylsulfonyl~L-cysteine. The yield was 95% for S-sulfo-Lcysteine and 9 1% for L-alanine 3-sullinic