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Enzymatic cleavage of fusion proteins using immobilised protease 3C

✍ Scribed by M. Hedhammar; H.R. Jung; S. Hober


Book ID
116828325
Publisher
Elsevier Science
Year
2006
Tongue
English
Weight
187 KB
Volume
47
Category
Article
ISSN
1046-5928

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## Abstract Addition of an N‐terminal fusion partner can greatly aid the expression and purification of a recombinant protein in __Escherichia coli__. We investigated two genetically engineered proteases designed to remove the fusion partner after the protein of interest has been expressed. Recombi