Enzymatic chemical transformation of rabbit insulin into human insulin
✍ Scribed by Yu. P. Shvachkin; S. P. Krasnoshchekova; A. M. Nikitina; S. M. Funtova; T. M. Anokhina; V. P. Fedotov; A. I. Ivanova
- Publisher
- Springer
- Year
- 1987
- Tongue
- English
- Weight
- 73 KB
- Volume
- 23
- Category
- Article
- ISSN
- 0009-3130
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## Abstract Peptide bond formation can be enzymatically catalysed by the reverse reaction of proteases. Application is seen in the industrial production of human insulin. Human insulin derivative can be enzymatically prepared using either porcine insulin or the single chain B(1–29)‐A(1–21) insulin
Summarv: Trypsin covalently bound to macroporous bead cellulose was found to have a sufficiently high activity to catalyze the direct conversion of porcine insulin into human insulin ethyl ester.