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Enzymatic characterization of CMP-NeuAc:Galβ1-4GlcNAc-R α(2-3)-sialyltransferase from human placenta

✍ Scribed by Martin Nemansky; Dirk H. Eijnden


Publisher
Springer US
Year
1993
Tongue
English
Weight
954 KB
Volume
10
Category
Article
ISSN
1573-4986

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Absorbance- and Light-Based Solid-Phase
✍ Jiunn-chern Yeh; Richard D. Cummings 📂 Article 📅 1996 🏛 Elsevier Science 🌐 English ⚖ 147 KB

We now report a solid-phase assay for CMPNeuAc: Galbeta1-3/4GlcNAc-R alpha-2,3-sialyltransferase (alpha2,3ST) that is nonradioactive and allows specific identification of the sialylated product. An acceptor glycoprotein, desialylated fetuin, is immobilized on a microtiter plate. The transfer of sial