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Enzymatic catalysis in gel-stabilized two-phase systems: improvement of the solvent phase

✍ Scribed by Bastien Doumèche; Matthias Heinemann; Jochen Büchs; Winfried Hartmeier; Marion B Ansorge-Schumacher

Book ID
114395754
Publisher
Elsevier Science
Year
2002
Tongue
English
Weight
210 KB
Volume
18
Category
Article
ISSN
1381-1177

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✍ Dong-Zhi Wei; Jian-Hang Zhu; Xue-Jun Cao 📂 Article 📅 2002 🏛 Elsevier Science 🌐 English ⚖ 77 KB

Cephalexin was synthesized in aqueous two-phase systems (ATPSs) by using penicillin G acylase (PGA) (penicillin amidohydrolase, EC3.5.1.11) as a catalyst and 7-aminodeacetoxicephalosporanic acid (7-ADCA) and phenylglycine methyl ester (PGME) as substrates. In the ATPS composed of 20% (w/w) PEG 400 a