In an enzymatic synthesis of cephalexin (CEX) using an acylase from Xanthomonas citri, the effect of polyethylene glycol (PEG) on the synthetic reaction of Zamino-3-deacetoxycephalosporanic acid (FADCA) and D-alpha-phenylglycine methyl ester (PGM) to CEX was investigated. The addition of PEG (MW 300
β¦ LIBER β¦
Enzymatic biosynthesis of cephalexin
β Scribed by D. K. Rhee; S. B. Lee; J. S. Rhee; Dewey D. Y. Ryu; J. Hospodka
- Publisher
- John Wiley and Sons
- Year
- 1980
- Tongue
- English
- Weight
- 385 KB
- Volume
- 22
- Category
- Article
- ISSN
- 0006-3592
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## Abstract A cascade of two enzymatic transformations is employed in a oneβpot synthesis of cephalexin. The nitrile hydratase (from __R. rhodochrous__ MAWE)βcatalyzed hydration of Dβphenylglycine nitrile to the corresponding amide was combined with the penicillin G acylase (penicillin amidohydrola