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Enzymatic assay for total plasma Cys

✍ Scribed by Han, Qinghong; Hoffman, Robert M

Book ID
109946533
Publisher
Nature Publishing Group
Year
2008
Tongue
English
Weight
201 KB
Volume
3
Category
Article
ISSN
1750-2799

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✍ Henry C. Poon; Bonnie M. Schmidt πŸ“‚ Article πŸ“… 1991 πŸ› Elsevier Science 🌐 English βš– 369 KB

An enzymatic fluorometric assay to quantitate plasma pyridoxal 5'-phosphate (PLP) is described. PLP is preincubated for 30 min with purified tyrosine decarboxylase apoenzyme (TDA) in acetate buffer and is then incubated with L-tyrosine for 60 min. The decarboxylated metabolite, tyramine, is extracte