Enzymatic assay for subnanomole amounts of l-fucose
β Scribed by John B. Morris
- Publisher
- Elsevier Science
- Year
- 1982
- Tongue
- English
- Weight
- 494 KB
- Volume
- 121
- Category
- Article
- ISSN
- 0003-2697
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β¦ Synopsis
An enzymatic assay capable of detecting subnanomole quantities of L-fucose has been developed. For this purpose, the NADH produced by the fucose dehydrogenase-catalyzed oxidation of L-fucose is analyzed by the enzymatic cycling technique of 0. H. Lowry, J. V.
π SIMILAR VOLUMES
Aldol reactions /
## Abstract Fucose is an important component of complex heterooligosaccharides. Here we describe the preparative enzymatic synthesis of ΓβLβfucoseβ1βphosphate as well as its enzymatic conversion into GDPβfucose. Fucose (1) was converted into ΓβLβfucoseβ1βphosphate (2) with fucose kinase (EC 2.7.1.5
## An ultrasensitive protein assay, linear between 0.01 and 0.2 pg protein, is described. In this assay, copper is complexed to protein, excess copper is removed by adsorption to a small Sephadex column, and the copper-protein complex is destroyed by digestion with hydroperoxide. Phenol and chlor