Enzymatic and chemical labelling of prostaglandin E1 and 13,14-dihydro-15-keto-prostaglandin E1

✍ Scribed by Dimitrios Tsikas; Stefan Bracht; Dirk Stichtenoth; Jürgen C. Frölich

• Publisher: John Wiley and Sons
• Year: 1993
• Tongue: French
• Weight: 447 KB
• Volume: 33
• Category: Article
• ISSN: 0022-2135
• DOI: 10.1002/jlcr.2580331208

✦ Synopsis

Abstract

Chemical and enzymatic tools were employed to synthesize [1,1‐^18^O~2~]‐prostaglandin (PG) E1, [1,1‐^18^O~2~]‐13,14‐dihydro‐15‐keto‐PGE~1~ and [5,6‐^3^H~2~]‐13,14‐dihydro‐15‐keto‐PGE~1~ starting from the corresponding unlabelled compounds adn [5,6‐^3^H~2~]‐PGE~1~, respectively. Reaction products were purified by high‐performance liquid chromatography and their isotopic purity was determined by gas chromatographymass spectrometry (GC‐MS). The utility of [1,1‐^18^O~2~]‐PGE~1~ and [1,1‐^18^O~2~]‐13,14‐dihydro‐15‐keto‐PGE~1~ as internal standards for GC‐MS analysis of the corresponding endogenous compounds in biological fluids is demonstrated. [5,6‐^3^H~2~]‐13,14‐dihydro‐15‐keto‐PGE~1~ was found to be useful in developing extraction and purification procedures for the quantitation of endogenous 13,14‐dihydro‐15‐keto‐PGE~1~ in human plasma by GC‐tandem MS.