Enzymatic alcoholysis of triolein to produce wax ester

✍ Scribed by Nursyamsyila Mat Hadzir; Mahiran Basri; Mohd Basyaruddin Abd Rahman; Che Nyonya Abdul Razak; Raja Noor Zaliha Abd Rahman; Abu Bakar Salleh

• Publisher: Wiley (John Wiley & Sons)
• Year: 2001
• Tongue: English
• Weight: 108 KB
• Volume: 76
• Category: Article
• ISSN: 0268-2575
• DOI: 10.1002/jctb.407

✦ Synopsis

Abstract

An alcoholysis reaction between triolein and oleyl alcohol catalyzed by Lipozyme and Novozyme was carried out to produce oleyl oleate, a wax ester. The effects of various reaction parameters such as time, reaction temperature, amount of enzyme, molar ratio of substrates (oleyl alcohol/triolein), various organic solvents used and the initial water activity, a~w~ of the reaction system were studied. The best conditions tested to produce wax ester were respectively, incubation time, 5 h; temperature, 50 °C for Lipozyme and 60 °C for Novozyme; weight of enzyme, 0.30 g and molar ratio of oleyl alcohol to triolein, 6:1. The use of organic solvents greatly influenced the activity of lipase. Generally, the activity of lipase was high in nonpolar solvents with log P values greater than 2.50. Heptane and hexane were the best solvents tested. The enzymatic synthesis of oleyl oleate was best carried out at a~w~ 0.32. Analysis of the yield of the products of the reaction at optimized reaction condition using Lipozyme showed that 75.66% oleyl oleate was produced.

© 2001 Society of Chemical Industry