Enzymatic alcoholyses of NAD: A New type of ADP-ribosylation reaction catalysed by NAD glycohydrolase
✍ Scribed by Tono-oka, Shuichi ;Azuma, Ichiro
- Book ID
- 102366870
- Publisher
- John Wiley and Sons
- Year
- 1997
- Tongue
- English
- Weight
- 416 KB
- Volume
- 1997
- Category
- Article
- ISSN
- 0947-3440
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✦ Synopsis
Abstract
As part of studies concerning the transglycosidation action of NAD glycohydrolase, the hydrolase‐catalysed reaction of normal alkyl alcohols (six alcohols from methanol to 1‐hexanol) has been investigated. All the alcoholic substrates were found to undergo ADP‐ribosylation with varying efficiences, to give the corresponding O‐ribosylated products (1–6). Yields (11–67%) were dependent upon the solubility of the substrates in aqueous media and upon their inhibitory effect on the enzyme activity. ^1^H NMR analyses demonstrated the alkyl β‐riboside structure of compounds 1–6 and the non‐equivalence of the O‐methylene protons of their alkoxy groups. This is the first report of characterization of O‐ribosylated products formed by the transglycosidation action of NAD glycohydrolase.