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Enzymatic activity of trypsin adsorbed on temperature-sensitive composite polymer particles

✍ Scribed by M. Okubo; H. Ahmad


Publisher
John Wiley and Sons
Year
1998
Tongue
English
Weight
156 KB
Volume
36
Category
Article
ISSN
0887-624X

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✦ Synopsis


Two kinds of temperature-sensitive composite polymer particles were prepared by seeded emulsion copolymerizations of (dimethylamino)ethyl methacrylate and ethylene glycol dimethacrylate with 0.14 mm-sized polystyrene and 0.26 mm-sized poly(methylmethacrylate) seed particles. To evaluate the usefulness as a carrier for biomolecules, the enzymatic activities of trypsin adsorbed on these two composite polymer particles were measured at temperatures above and below each lower critical solution temperature (LCST). In both cases, adsorbed trypsin retained its enzymatic activity during repeated adsorption/desorption measurements.


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## SYNOPSIS Core-shell composite polymer particles having temperature-sensitive shell were prepared by seeded emulsion copolymerization of dimethylaminoethyl methacrylate and ethylene glycol dimethacrylate (EGDM) with 0.14 pm-sized polystyrene seed particles according to our previous article. In t