Enzymatic activities and infrared studies of glutamate dehydrogenase immobilized on Langmuir-Blodgett films

✍ Scribed by Marie-Laure Hourdou; Françoise Besson

• Publisher: Springer-Verlag
• Year: 1995
• Tongue: English
• Weight: 445 KB
• Volume: 9
• Category: Article
• ISSN: 0951-208X
• DOI: 10.1007/bf00156349

✦ Synopsis

A technique is described for the immobilization of active glutamate dehydrogenase (GDH) on behenic acid Langmuir-Blodgett (LB) films. The optimization of the immobilization conditions shows that the activities of GDH bound on hydrophobic and hydrophilic LB films were similar and decreased dramatically when the immobilized enzyme was dried. The GDH binding was followed by Fourier transform infrared (FTIR) spectroscopy. Modifications of GDH conformation and LB film structure were observed during the enzyme binding. After GDH activity test, a partial dissociation of behenic acid occurred and the B-sheet band of the enzyme increased by comparison with the a-helix band.