✦ LIBER ✦
Enumeration ofDrosophilaform II DNA-dependent RNA polymerase initiation sites onDrosophilaDNA
✍ Scribed by John P. Phillips
- Book ID
- 104783997
- Publisher
- Springer
- Year
- 1979
- Tongue
- English
- Weight
- 425 KB
- Volume
- 17
- Category
- Article
- ISSN
- 0006-2928
No coin nor oath required. For personal study only.
✦ Synopsis
The in vitro incorporation of gamma-32P-labeled nucleoside triphosphates into RNA by Drosophila melanogaster form II RNA polymerase from template sites which afford protection from the initiation inhibitor, polyriboinosinic acid (poly [I]), is used as a method for enumerating a specific class of transcription initiation sites on D. melanogaster DNA. Such sites number about 4000 per haploid genome for D. melanogaster. This value is in good agreement with the number of functional genetic units in the D. melanogaster genome as determined by classical cytogenetics.