The need for a rapid and reliable marker for the engraftment potential of hematopoietic stem and progenitor cell (HPC) transplants has led to the development of flow cytometric assays to quantitate such cells on the basis of their expression of CD34. The variability associated with enumeration of lo
Enumeration of CD34+ hematopoietic stem cells for reconstitution following myeloablative therapy
β Scribed by R. A. Roscoe; W. B. Rybka; A. Winkelstein; A. M. Houston; J. E. Kiss
- Publisher
- John Wiley and Sons
- Year
- 1994
- Tongue
- English
- Weight
- 633 KB
- Volume
- 16
- Category
- Article
- ISSN
- 0196-4763
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Expansion of hematopoietic stem cells (HSCs) from cord blood is highly desired for treatment and transplantation of adult patients for hematologic diseases. For efficient proliferation of HSCs, CD34(+) cells from cord blood were co-cultured with microencapsulated murine stromal cells (HESS-5) or imm
## Abstract ## Background Characterization of hematopoietic stem cells (HSCs) by laser scanning cytometry (LSC) was compared with conventional flow cytometry (FCM). The method was evaluated for application in the development of advanced cell culture substrates that were supposed to support the ex
The flow cytometric enumeration of CD34+ hemopoietic precursor cells (HPC) present in samples used for transplantation of HPC has proven to be the most powerful single parameter for prediction of engraftment. At present, several different methodological approaches are used for the flow cytometric en