Entrapment of chloroplasts and thylakoids in polyvinylalcohol-SbQ. Optimization of membrane preparation and storage conditions
✍ Scribed by R. Rouillon; J.-J. Mestres; J.-L. Marty
- Publisher
- Elsevier Science
- Year
- 1995
- Tongue
- English
- Weight
- 535 KB
- Volume
- 311
- Category
- Article
- ISSN
- 0003-2670
No coin nor oath required. For personal study only.
✦ Synopsis
Chloroplasts and thylakoids isolated from spinach were entrapped in six different polyvinylalcohol bearing styrylpyridium groups (PVA-SbQ). Measurements of oxygen production were carried out in 0.01 M phosphate buffer (pH 6) at 25" C. I~ob~~~ preparations were compared for their electron transport activity measured as the initial rate of oxygen evolution with p-beuzoquinone as artificial electron acceptor. The entrapped chloroplasts in PVA-SbQ 1700 and 3500 betaine retained 20% of their initial activity after 90-day storage in the dark at +4" C. A membrane made with PVA-SbQ 2300 kept 20% activity after 427day storage at -18" C in the dark whereas the same membrane retained only 10% activity after !&day storage at + 4" C in the dark. A 1% BSA ~ncen~ation increased the stab~ity of the membrane. The limits of detection (corresponding to a 10% inhibition) of atrazine were 10 pgfl and 12 pgfl for native and entrapped chloroplasts, respectively. The good stability of entrapped chloroplasts suggests that this system could be useful for the detection of pollutants in water,