Entrapment of atrazine-degrading enzymes in sol-gel glass
โ Scribed by Carl G. Kauffmann; Raphi T. Mandelbaum
- Book ID
- 119524640
- Publisher
- Elsevier Science
- Year
- 1996
- Tongue
- English
- Weight
- 630 KB
- Volume
- 51
- Category
- Article
- ISSN
- 0168-1656
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Sol-gel entrapment was evaluated as a method for immobilization of an atrazine degrading Pseudomonas. It was found that the bacterium lost much of its atrazine degrading activity upon immobilization. However, partial activity could have been restored by amendment of nutrients. Bacteria immobilized u
We report the successful doping of a sol-gel matrix with an antibody, retaining its ability to bind free antigen from an aqueous solution. The particular system described is monoclonal anti-atrazine mouse antibody which was entrapped in SiOz sol-gel matrices, prepared from tetramethoxysilane by seve
The present work reports on the entrapment of parathion hydrolase from Pseudomonas spp. into the sol-gel glass matrix. Enzyme entrapment was studied in the range of 0.01-0.25 U, and compared with the activity of the free, non-immobilized enzyme. The reaction catalyzed by the entrapped enzyme was alm