Entire litters developed from transferred eggs in whole body X-irradiated female mice
✍ Scribed by Lin, T. P.
- Book ID
- 102336220
- Publisher
- John Wiley and Sons
- Year
- 1980
- Tongue
- English
- Weight
- 362 KB
- Volume
- 213
- Category
- Article
- ISSN
- 0022-104X
No coin nor oath required. For personal study only.
✦ Synopsis
Abstract
The sensitivity of mouse eggs to sublethal X‐irradiation was determined in vitro and in vivo with regard to the development of donor litters in foster mothers. One thousand seven hundred fifty‐eight unfertilized eggs of agouti dark‐eyed donor mice were transferred into 293 unirradiated or X‐irradiated, mated female pink‐eyed mice. Two hundred thirty‐nine recipients became pregnant; of these 35 produced litters containing solely dark‐eyed fetuses. Sublethal doses of X‐radiation administered to donor eggs in vitro before transferring into unirradiated recipients did not influence significantly the number of litters of exclusively dark‐eyed fetuses produced. However, recipients irradiated by 250 roentgens (r) produced more solely dark‐eyed litters than did those irradiated with 100 r. In 21 pregnant females irradiated by 100 r, only 3 (14%) developed solely dark‐eyed fetuses as compared to 22 pregnant females irradiated by 250 r, of which 13 (59%) developed solely dark‐eyed fetuses, all from unirradiated, transferred eggs. Of another group of 22 pregnant females which received 250 r body irradiation and subsequently received eggs also irradiated by 250 r, only 7 (32%) produced litters of dark‐eyed fetuses. No one female of these three groups carried native fetuses. Such radiation‐induced infertility resulting from damage of native eggs rather than loss of the mother's ability to carry a pregnancy, is frequently remedied by egg transfer.