Enthalpimetric flow analysis of urea using immobilized urease
β Scribed by Richard K. Owusu; Martin J. Trewhella; Arthur Finch
- Book ID
- 103413197
- Publisher
- Elsevier Science
- Year
- 1987
- Tongue
- English
- Weight
- 437 KB
- Volume
- 111
- Category
- Article
- ISSN
- 0040-6031
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Serum samples were analyzed for their urea content using fluorescence flow injection analysis incorporating an immobilized urease bioreactor and a gas permeable separator. The urease was immobilized under mild and facile conditions to a hydrophilic 2-fluoro-1-methylpyridinium-activated support. The
## Abstract The immobilization of urease on the reticulated polyurethane foam, and the kinetic phenomenon of urea hydrolysis by the resulting immobilized urease in both batch squeezer and circulated flow reactors were studied. Urease was immobilized with bovine serum albumin and glutaraldehyde on p