Enrichment of suppressor cell activity by hydrocortisone: Suppression of in vitro activation of splenocytes from mice bearing lewis lung carcinoma

✍ Scribed by Enrico Clerici; Bilha Schechter; Michael Feldman

• Publisher: John Wiley and Sons
• Year: 1980
• Tongue: French
• Weight: 594 KB
• Volume: 25
• Category: Article
• ISSN: 0020-7136
• DOI: 10.1002/ijc.2910250308

✦ Synopsis

Abstract

Suppressor cells in spleens of mice bearing the Lewis lung carcinoma (3LL) suppressed the anti‐tumor immune response in vivo but not in vitro. The hydrocortisone (HC)‐resistant fraction of spleen cells from tumor‐bearing mice (TBM) was, on the other hand, suppressive in vitro, due to enrichment for HC‐resistant suppressor cells. This cellular fraction suppressed the tumor‐induced differentiation of memory cells as well as the tumor‐independent in vitro activation of TBM spleen cells. The in vitro lymphoproliferative response of TBM spleen cells to mitogens was also subjected to suppression: these cells showed a lower response to a mitogen such as Concanavalin A (Con A) and were capable of suppressing the response of normal spleen cells to this mitogen. HC treatment of TBM 2 days prior to spleen harvest enriched the splenic population for suppressor cells that suppressed Con A‐induced activation.