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Enrichment of mutants of Rhodosporidium toruloides by the use of inositol starvation

✍ Scribed by Dr. M. Tully

Publisher
John Wiley and Sons
Year
1985
Tongue
English
Weight
289 KB
Volume
25
Category
Article
ISSN
0233-111X

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✦ Synopsis

Inositol-requiring mutant,s of strains of Rhodosporidium toruloides were isolated and the frequency of isolation of auxotrophic and catabolic mutants from them was measured after starvation for inositol. Mutants can be isolated a t frequencies up to 31 times greater than those of nintagenized cultures without enrichment.

πŸ“œ SIMILAR VOLUMES

Selection of spontaneous mutants by inos
Selection of spontaneous mutants by inositol starvation in yeast
✍ Henry, Susan A. ;Donahue, Thomas F. ;Culbertson, Michael R. πŸ“‚ Article πŸ“… 1975 πŸ› Springer 🌐 English βš– 658 KB

A new method for the routine isolation of mutations of spontaneous origin in the yeast, Saccharomyces cerevisiae, is reported. The technique is based on the observation that inositol auxotrophs die when deprived of inositol. However, if macromolecular synthesis is inhibited, most of the cells surviv