β Scribed by J. Wiegel; H. G. Schlegel
No coin nor oath required. For personal study only.
An enrichment method for nitrogen fixing hydrogen bacteria is described. The procedure invariably resulted in the isolation of yellow-pigmented coryneform bacterial strains assigned to Corynebacterium autotrophicum. The procedure included a serial transfer in an ammonium-free mineral liquid medium under an atmosphere of 10% hydrogen, 5% oxygen, 10% carbon dioxide and 75% nitrogen, followed by a short alkali treatment and by streaking on nutrient broth-succinate agar. The ability to fix nitrogen was confirmed by the acetylene reduction test and by 15N2 incorporation.
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Twenty out of 400 isolates of bacteria mainly from salt marshes and saline soils of Egypt were successfully grown on mannitol N-free medium. The nitrogen-fixing activity was then demonstrated for the twenty isolates in modified STANIER'S medium using the acetylene reduction assay. All of them posses
The primary steps of N2, ammonia and nitrate metabolism in Klebsiella pneumoniae grown in a continuous culture are regulated by the kind and supply of the nitrogenous compound. Cultures growing on N2 as the only nitrogen source have high activities of nitrogenase, unadenylated glutamine synthetase a