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Enhancing the specificity of the enterokinase cleavage reaction to promote efficient cleavage of a fusion tag

✍ Scribed by S. Hesam Shahravan; Xuanlu Qu; I-San Chan; Jumi A. Shin


Book ID
116828787
Publisher
Elsevier Science
Year
2008
Tongue
English
Weight
355 KB
Volume
59
Category
Article
ISSN
1046-5928

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We have studied the cleavage efficiency of the protease enterokinase (EK) using the novel vector pESP4. pESP4 is a yeast expression vector equipped with ligation-independent cloning sites, a GST purification tag, and a FLAG epitope tag. EK is used to cleave the FLAG and GST tags leaving the protein