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Enhancing catalytic activity of a hybrid xylanase through single substitution of Leu to Pro near the active site

✍ Scribed by Qian Wang; Li-Li Zhao; Jian-Yi Sun; Jian-Xin Liu; Xiao-Yan Weng


Book ID
106565825
Publisher
Springer
Year
2011
Tongue
English
Weight
336 KB
Volume
28
Category
Article
ISSN
1573-0972

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The gene encoding an esterase (PsyEst) of Psychrobacter sp. Ant300, a psychrophilic bacterium isolated from Antarctic soil, was cloned, sequenced, and expressed in Escherichia coli. PsyEst, which is a member of hormone-sensitive lipase (HSL) group of the lipase/esterase family, is a cold-active, the