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Enhancement of tryptophan production byEscherichia colias an application of genetic engineering

✍ Scribed by Shuichi Aiba; Tadayuki Imanaka; Hiroshi Tsunekawa

Publisher
Springer Netherlands
Year
1980
Tongue
English
Weight
373 KB
Volume
2
Category
Article
ISSN
0141-5492

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✦ Synopsis

A feedback resistant trp operon plasmid that transformed a multiple mutant (W@? WA) of Escherichia coZi was found to enhance remarkably the production of tryptophan in a bench-scale fermentor. 5.5 g of tryptophan was accumulated per litre of culture medium at 24th hr in batch. The productivity was 0.229 g/R/hr. This productivity is the highest among those ever reported by other workers. The recombinant plasmid (Tcr Trp+ I-) used was completely stable in each run when tetracycline was added by 10 ug/R into the medium.

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