Enhancement of transdermal absorption, gene expression and stability of tyrosinase plasmid (pMEL34)-loaded elastic cationic niosomes: Potential application in vitiligo treatment
✍ Scribed by Jiradej Manosroi; Narinthorn Khositsuntiwong; Worapaka Manosroi; Friedrich Götz; Rolf G. Werner; Aranya Manosroi
- Publisher
- John Wiley and Sons
- Year
- 2010
- Tongue
- English
- Weight
- 328 KB
- Volume
- 99
- Category
- Article
- ISSN
- 0022-3549
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✦ Synopsis
The pMEL34 was loaded in elastic cationic niosomes (Tween61/Cholesterol/DDAB at 1:1:0.5 molar ratio) by chloroform film method with sonication and rehydrated with 25% ethanol. The amount of pMEL34 was determined by gel electrophoresis and gel documentation. The maximum loading of pMEL34 in elastic cationic niosomes was 150 mg/16 mg of the niosomal compositions. At 8 weeks, the remaining plasmid in the elastic niosomes kept at 4 AE 28C, 27 AE 28C were 49.75% and 38.57%, respectively, whereas at 45 AE 28C, all plasmids were degraded. For transdermal absorption through rat skin investigated by Franz diffusion cells at 6 h, the fluxes of pMEL34 loaded in elastic and nonelastic niosomes in viable epidermis and dermis (VED) were 0.022 AE 0.00 and 0.017 AE 0.01 mg/cm 2 /h, respectively, whereas only pMEL34 loaded in elastic cationic noisome was observed in the receiver solution. The pMEL34 loaded in elastic cationic niosomes showed the highest tyrosinase gene expression demonstrating higher tyrosinase activity than the free and the loaded plasmid in nonelastic niosomes of about four times. This study has suggested the potential application of elastic cationic niosomes as an efficient topical delivery for tyrosinase gene in vitiligo therapy.