Enhancement of the Antibody-dependent Tumoricidal Activity of Human Monocytes by Human Monocytic Colony-stimulating Factor
β Scribed by Suzu, Shinya ;Yanai, Nobuya ;Saito, Minoru ;Kawashima, Takuji ;Kasahara, Tadashi ;Saito, Masaki ;Takaku, Fumimaro ;Motoyoshi, Kazuo
- Book ID
- 108578995
- Publisher
- Wiley (Blackwell Publishing)
- Year
- 1990
- Tongue
- English
- Weight
- 367 KB
- Volume
- 81
- Category
- Article
- ISSN
- 0910-5050
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## Abstract Monocytes were separated from peripheral blood mononuclear cells of normal human donors by adherence on plastic conditioned by cell lines (microexudatecoated plastic) and harvested by exposure to ethylene diamine tetraβacetic acid. Cytolytic activity was tested by incubating effector ce
Human peripheral blood mononuclear cells (lymphocytes and monocytes) were preincubated for 0-24 h with human recombinant granulocyte-monocyte-colony-stimulating factor (GM-CSF) and used as effector cells in an 18 h antibody-dependent cellular cytotoxicity (ADCC) assay with SW948 (a human colorectal