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Enhancement of shoot regeneration from cotyledon explants ofBrassica rapasspoleiferathrough pretreatment with auxin and cytokinin and use of ethylene inhibitors

✍ Scribed by Laurie Burnett; Maryanne Arnoldo; Stephen Yarrow; Bin Huang


Book ID
104619866
Publisher
Springer
Year
1994
Tongue
English
Weight
268 KB
Volume
37
Category
Article
ISSN
0167-6857

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✦ Synopsis


The presence of benzyladenine or naphthaleneacetic acid in seed germination medium markedly enhanced subsequent shoot regeneration from the base of the excised cotyledon explants of Brassica rapa cv. Horizon. Cotyledon explants from younger seedlings (3 or 4-day old) produced more shoots than those from older seedlings. Addition of the ethylene inhibitor aminoethoxyvinylglycine (1.0 ~tM) to the regeneration medium improved shoot regeneration three fold.