Enhancement of proliferation in cultures of Chinook salmon embryo cells by interactions between inosine and bovine sera
✍ Scribed by R. C. Ganssin; Q. H. Tran; T. F. Rabgey; N. C. Bols
- Book ID
- 102885168
- Publisher
- John Wiley and Sons
- Year
- 1994
- Tongue
- English
- Weight
- 932 KB
- Volume
- 160
- Category
- Article
- ISSN
- 0021-9541
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✦ Synopsis
Abstract
The influence of inosine on DNA synthesis by Chinook salmon embryo cells (CHSE‐214) was investigated because previously cell number was shown to increase from six‐ to thirtyfold if inosine was added to the basal medium (L‐15) supplemented with either dialyzed fetal bovine serum (dFBS), calf serum (CS), or dCS. Relative to L‐15, ^3^H‐thymidine incorporation was inhibited by these sera alone but elevated in nondialyzed (intact) FBS. Inosine at 10 μM stimulated ^3^H‐thymidine incorporation from ten‐ to seventyfold in dFBS, CS, and dCS but was only slightly stimulatory in FBS and in L‐15 alone. As well as inosine, hypoxanthine, cIMP, IMP, IDP, and ITP were just as stimulatory, but the nonsalvageable purines (xanthine, xanthosine, and XMP) were not. The stimulatory action of inosine was highest in low density cultures. Dipyridamole and S‐(p‐nitrobenzyl)‐6‐thioinosine (NBTI), inhibitors of facilitated nonconcentrative nucleoside transport, did not completely block the enhancement of cell number by inosine and by themselves increased proliferation in CS and dCS. Overall, these results suggest that exogenous inosine promoted CHSE‐214 proliferation by overcoming factors in the nondialyzable fraction of sera that led to purine loss and by raising intracelular purine nucleotides to levels necessary for cells to respond to growth factors in the nondialyzable fraction of sera. © 1994 Wiley‐Liss, Inc.