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Enhancement of production and regeneration of embryogenic type II callus inZea maysL. by AgNO3

✍ Scribed by Philippe Vain; Horth Yean; Pascal Flament

Publisher: Springer
Year: 1989
Tongue: English
Weight: 745 KB
Volume: 18
Category: Article
ISSN: 0167-6857
DOI: 10.1007/bf00047740

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✦ Synopsis

In order to evaluate the impact of ethylene in maize tissue culture, silver nitrate has been used as an inhibitor of ethylene action. Type II callus initiation rate was improved when immature embryos were cultured on a modified Murashige & Skoog medium containing various concentrations of silver nitrate (5, 10, 20 mg 1-J ). Regeneration ability of calli initiated and maintained in presence of silver nitrate was enhanced. No modification of callus growth rate neither of ethylene production has been detected.

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AgNO3increases type II callus production from immature embryos of maize inbred B73 and its derivatives

✍ D. D. Songstad; C. L. Armstrong; W. L. Petersen 📂 Article 📅 1991 🏛 Springer 🌐 English ⚖ 421 KB

Incorporating 10 to 100 μM AgNO3 into Phytagel(™) (0.2%) solidified N6 medium containing 1 mg/L 2,4-D, 100 mg/L casamino acids and 25 mM praline (N6 1-100-25) promoted type II callus production from cultured Zea mays L. immature embryos of FRB73, B73 X A188 and a proprietary B73 BC6 genotype. Under