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Enhancement of phenotypic expression in cultured malignant liver epithelial cells by a complex medium

✍ Scribed by J. Martyn Gunn; Hisashi Shinozuka; Gary M. Williams

Publisher
John Wiley and Sons
Year
1976
Tongue
English
Weight
902 KB
Volume
87
Category
Article
ISSN
0021-9541

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✦ Synopsis

Abstract

The ability of two media identically supplemented with serum to maintain functional and structural differentiated properties of cultured malignant liver epithelial cells was compared. Reuber H35 hepatoma cells grown in a complex medium, Medium E, possessed the gluconeogenic enzyme phosphoenolpyruvate carboxykinase and bile canaliculi. When grown in Eagle's Minimal Essential Medium, the activity of phosphoenolpyruvate carboxykinase was reduced and bile canaliculi were not formed. This decrease in properties was restored when cells maintained in Minimal Essential Medium were returned to Medium E. It was concluded that the enhanced phenotypic expression was probably due to enrichment of the nutrient environment.

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